Виділення антибактеріального нано-гідроксиапатитного біоматеріалу з кісток буйволів та його характеристика

Kshama Parajuli1,Komal Prasad Malla1,2 ,Nicodemus Panchen1, Ganga G.C.3, Rameshwar Adhikari1,2
Affiliation: 
1Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal 2Research Centre for Applied Science and Technology (RECAST), Tribhuvan University, Kirtipur, Kathmandu, Nepal 3Department of Microbiology, Tri-Chandra M. Campus, Ghantaghar, Kathmandu, Nepal nepalpolymer@yahoo.com
DOI: 
https://doi.org/10.23939/chcht16.01.133
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Abstract: 
Методом термічного розкладу з біовідходів (кісток буйвола) виділені наночастинкигідроксиапатиту (HAp). Отриманий білий порошкоподібний матеріал охарактеризований за допомогою інфрачервоної спектроскопії з перетворенням Фур’є (FTIR), дифракції рентгенівських променів (XRD), скануючої електронної мікроскопії (SEM) та енергодисперсійного рентгенівського аналізу (EDX). За допомогою FTIR підтверджено, що термічне оброблення кісткового порошку при температурі 1223 К або вище видаляє органічні частини, що призводить до утворення чистого неорганічного біомінералу. Рентгенофазовий аналіз показав, що отриманий матеріал єнанокристалічнимHAp (nano-HAp) із середнім діаметром зерен 25 нм, а їх паличкоподібні частинки з щільно агломерованою морфологією підтверджені аналізом SEM. Крім кальцію (Ca), фосфору (P) і кисню (O), слідові кількості алюмінію (Al), магнію (Mg), міді (Cu), цирконію (Zr) і вуглецю (C) виявлені за допомогою EDX. Антибактеріальну активність nano-HAp проти шести стандартних штамів досліджували методом дифузії. В інтервалі досліджуваних концентрацій встановлено, що найбільшу активність nano-HAp виявляє доAcinetobacterbaumannii, меншу активність доEscherichiacoli, Pseudomonasaeruginosa та Staphylococcusaureus, і зовсім неактивний щодо Salmonellatyphi та Staphylococcusaureus, стійкого до метициліну (MRSA).Показано, щонано-HAp потенційно можна застосовувати в біомедицині.
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