Дослідження властивостей, антиоксидантна актив-ність та in silico молекулярний докінг хітозану з відходів черепашок равлика за допомогою ультра-звукової методикии
Affiliation:
1 Doctoral student of Biology, Faculty of Mathematic and Natural Sciences, University of Brawijaya, Malang 65145, East Java, Indonesia.
2 Department of Pharmacy, Diploma III Pharmacy, Academy Pharmacy of Surabaya, Surabaya 60231, East Java, Indonesia.
3 Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang 65145, East Java, Indonesia.
4 Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang 65145, East Java, Indonesia.
5 Essential Oil's Institute, University of Brawijaya, Malang 65145, East Java, Indonesia.
umarudin@student.ub.ac.id
DOI:
https://doi.org/10.23939/chcht17.01.126
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Abstract:
Равлики поширені в Індонезії, особливо в Кедірі, але черепашки равлика не мають комерційної цінності. У цій роботі описано характеристику й in vitro оцінку біоактивності хітозану з відходів черепашок равликів (хітозан-SSW), отриманого за допомогою ультразвукової методики, та проаналізовано потенціал хітозану як інгібітора рецепторів вільних радикалів за допомогою методу молекулярного докінгу in silico. Мета дослідження властивостей хітозану-SSW – аналіз вмісту води, білка та функціональних груп, а також молекулярної маси, розміру частинок, морфології, оцінки антиоксидантної активностімолекулярного докінгу in silico. Встановлено, що хітозан-SSW, отриманий за допомогою ультразвукової обробки, мав високий ступінь деацетилювання (DD) і високу молекулярну масу (MW). Встановлено характеристики хітозану-SSW: вміст води 0,43 %, вміст білка 1,59 %, молекулярна маса 2198 кДа, значення ступеня деацетилювання 79,50 %. Важливо, що хітозан-SSW мав високу антиоксидантну активність для потенційного зменшення вільних радикалів DPPH зі значенням IC50 2,44мкг/мл. Передбачається, що хітозан має потенціал як інгібітор ліпоксигенази, CYP2C9 і NADPH-оксидази
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