Error message

  • Deprecated function: Unparenthesized `a ? b : c ? d : e` is deprecated. Use either `(a ? b : c) ? d : e` or `a ? b : (c ? d : e)` in include_once() (line 1439 of /home/science2016/public_html/includes/bootstrap.inc).
  • Deprecated function: Array and string offset access syntax with curly braces is deprecated in include_once() (line 3557 of /home/science2016/public_html/includes/bootstrap.inc).

Phytochemical Screening, Antibacterial and Antioxidant Activities of Ocimum basilicum L. Cultivated in Biskra, Algeria

Fettah Asma1, Hachani Salah Eddine2, Chennai Yassmine1, Zeghdoud Hanane1
Affiliation: 
1 Laboratory of Molecular Chemistry and Environment (LMCE), University of Biskra, BP 145, 07000 Biskra, Algeria 2 Laboratory of Applied Chemistry (LCA), University of Biskra, BP 145, 07000 Biskra, Algeria a.fettah@univ-biskra.dz, asmafettah@yahoo.fr
DOI: 
https://doi.org/10.23939/chcht17.02.397
AttachmentSize
PDF icon full_text.pdf959.55 KB

Keywords:

Abstract: 
Algerian flora contains a wide range of aromatic plants of great therapeutic interest thanks to their biologically active secondary metabolites which makes them a subject of scientific interest. In this work, we were interested in Ocimum basilicum L. of Lamiaceae family cultivated and harvested in Biskra city located at South-East of Algeria. Phytochemical screening has been per-formed to reveal the presence of flavonoids, tannins, coumarins, essential oil, and other phytochemicals. The contents of total polyphenols, total flavonoids and total tannins have been determined. The obtained essential oil and extracts have been the subject of antibacterial and antioxidant assays. Our outcomes confirmed that Ocimum basilicum L. has a multiplicity of potential phytochemicals with a considerable amount and can be used as an alternative natural source of antioxidant and antibacterial components.
References: 

[1] Karkowska-Kuleta, J.; Rapala-Kozik, M.; Kozik, A. Fungi Pathogenic to Humans: Molecular Bases of Virulence of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. Acta Biochim. Pol. 2009, 56, 211-224. https://doi.org/10.18388/abp.2009_2452
https://doi.org/10.18388/abp.2009_2452

[2] Ajaib, M.; Almas, M.; Khan, K. M.; Perveen, S.; Shah, S. Phytochemical Screening, Antimicrobial and Antioxidant Activities of Ficus natalensis. J. Chem. Soc. Pak. 2016, 38, 345-351.

[3] Maxwell, S.R.J. Prospects for the Use of Antioxidant Therapies. Drugs 1995, 49, 345-361. https://doi.org/10.2165/00003495-199549030-00003
https://doi.org/10.2165/00003495-199549030-00003

[4] Ramarathnam, N.; Osawa, T.; Ochi, H.; Kawakishi, S. The Contribution of Plant Food Antioxidants to Human Health. Trends Food Sci. Technol. 1995, 6, 75-82. https://doi.org/10.1016/S0924-2244(00)88967-0
https://doi.org/10.1016/S0924-2244(00)88967-0

[5] Jean, B. Pharmacognosie, phytochimie, plantes médicinales, 4e éd. ; Lavoisier, 2009.

[6] Vorobyova, V.; Shakun, A.; Chygyrynets O.; Skiba, M.; Julia Zaporozhets, J. Antioxidant Activity and Phytochemical Screening of the Apricot Cake Extract: Experimental and Theoretical Studies. Chem. Chem. Technol. 2020, 14, 372-379. https://doi.org/10.23939/chcht14.03.372
https://doi.org/10.23939/chcht14.03.372

[7] Dupond, F.; Guignard, J. Botanique: Les familles de plantes , 15e édition. sl. ; Elsevier Masson: Paris, 2012.

[8] Umerie, S.C.; Anaso, H.U.; Anyasoro, L.J.C. Insecticidal Potentials of Ocimum basilicum Leaf-Extract. Bioresour. Technol. 1998, 64, 237-239. https://doi.org/10.1016/S0960-8524(97)00188-0
https://doi.org/10.1016/S0960-8524(97)00188-0

[9] Pascual-Villalobos, M.J.; Ballesta-Acosta, M.C. Chemical Variation in an Ocimum basilicum Germplasm Collection and Activity of the Essential Oils on Callosobruchus maculatus. Biochem. Syst. Ecol. 2003, 31, 673-679. https://doi.org/10.1016/S0305-1978(02)00183-7
https://doi.org/10.1016/S0305-1978(02)00183-7

[10] Govindarajan, M.; Sivakumar, R.; Rajeswary, M.; Yoga-lakshmi, K. Chemical Composition and Larvicidal Activity of Es-sential oil from Ocimum basilicum (L.) against Culex tritaeniorhyn-chus, Aedes albopictus and Anopheles subpictus (Diptera: Culici-dae). Exp. Parasitol. 2013, 134, 7-11. https://doi.org/10.1016/j.exppara.2013.01.018
https://doi.org/10.1016/j.exppara.2013.01.018

[11] Ekren, S.; Sönmez, Ç.; Özçakal, E.; Kurttaş, Y. S. K.; Bay-ram, E.; Gürgülü, H. The Effect of Different Irrigation Water Levels on Yield and Quality Characteristics of Purple Basil (Ocimum basilicum L.). Agric. Water Manag. 2012, 109, 155-161. https://doi.org/10.1016/j.agwat.2012.03.004
https://doi.org/10.1016/j.agwat.2012.03.004

[12] Benedec, D.; Oniga, I.; Oprean, R.; Tamas, M. Chemical Composition of the Essential Oils of Ocimum basilicum L. Culti-vated in Romania. Farmacia 2009, 57, 625-629.

[13] Kusuma, H.; Putri, D.; Dewi, I.; Mahfud, M. Solvent-free Microwave Extraction as the Useful Tool for Extraction of Edible Essential Oils. Chem. Chem. Technol. 2016, 10, 213-218. https://doi.org/10.23939/chcht10.02.213
https://doi.org/10.23939/chcht10.02.213

[14] Kusuma, H.; Putri, D.; Dewi, I.; Mahfud, M. Solvent-Free Microwave Extraction of Essential Oil from Dried Basil (Ocimum basilicum L.) Leaves. Chem. Chem. Technol. 2018, 12, 543-548. https://doi.org/10.23939/chcht12.04.543
https://doi.org/10.23939/chcht12.04.543

[15] Detraz, P. Catalogue Des Basilics ; Jardin Des Saveurs, 2001.

[16] MacKee, H.S. Catalogue des plantes introduites et cultivées en Nouvelle-Calédonie ; Paris, 1994.

[17] Elyemni, M.; Louaste, B.; Nechad, I.; Elkamli, T.; Bouia, A.; Taleb, M.; Chaouch, M.; Eloutassi, N., Extraction of Essential Oils of Rosmarinus officinalis L. by Two Different Methods: Hydrodis-tillation and Microwave Assisted Hydrodistillation. Sci. World J. 2019, 2019, 3659432. https://doi.org/10.1155/2019/3659432
https://doi.org/10.1155/2019/3659432

[18] Wesolowska, A.; Grzeszczuk, M.; Jadczak, D. Comparison of the Chemical Composition of Essential Oils Isolated by Water-steam Distillation and Hydrodistillation from Garden Thyme (Thy-mus vulgaris L.). J. Essent. Oil Bear. Pl. 2016, 19, 832-842. http://dx.doi.org/10.1080/0972060X.2015.1025296
https://doi.org/10.1080/0972060X.2015.1025296

[19] Liyana-Pathirana, C.M.; Shahidi, F. Importance of Insoluble-Bound Phenolics to Antioxidant Properties of Wheat. J. Agric. Food Chem. 2006, 54, 1256-1264. https://doi.org/10.1021/jf052556h
https://doi.org/10.1021/jf052556h

[20] EL-Haoud, H.; Boufellous, M.; Berrani, A; Tazougart, H.; Bengueddour, R. Screening phytochimique d'une plante medicinale: Mentha Spicata L. Am. J. innov. res. appl. sci. 2018, 7, 226-233.

[21] http://dspace.univ-tlemcen.dz/.../Contribution-a-l-etude-de-%20plantes-%... (accessed Sep 14, 2019).

[22] Ribéreau-Gayon, P. Les Composés phénoliques des végétaux: par Pascal Ribéreau-Gayon ; Dunod, 1968.

[23] Boizot, N.; Charpentier, J.-P. Méthode rapide d'évaluation du contenu en composés phénoliques des organes d'un arbre forestier. Cahier des Techniques de l'INRA 2006, 79-82.

[24] Škerget, M.; Kotnik, P.; Hadolin, M.; Hraš, A. R.; Simonič, M.; Knez, Ž., Phenols, Proanthocyanidins, Flavones and Flavonols in Some Plant Materials and their Antioxidant Activities. Food Chem. 2005, 89, 191-198. https://doi.org/10.1016/j.foodchem.2004.02.025
https://doi.org/10.1016/j.foodchem.2004.02.025

[25] Dewanto, V.; Wu, X.; Adom, K.K.; Liu, R.H. Thermal Processing Enhances the Nutritional Value of Tomatoes by Increas-ing Total Antioxidant Activity. J. Agric. Food Chem. 2002, 50, 3010-3014. https://doi.org/10.1021/jf0115589
https://doi.org/10.1021/jf0115589

[26] Ayoola, G.A.; Ipav, S.S.; Sofidiya, M.O.; Adepoju-Bello, A.A.; Coker, H.A.; Odugbemi, T.O. Phytochemical Screening and Free Radical Scavenging Activities of the Fruits and Leaves of Allanblackia floribunda Oliv (Guttiferae). Int. J. Health Res. 2008, 1, 87-93. https://doi.org/10.4314/ijhr.v1i2.47920
https://doi.org/10.4314/ijhr.v1i2.47920

[27] Schofield, P.; Mbugua, D.M.; Pell, A.N. Analysis of Con-densed Tannins: A Review. Anim. Feed Sci. Technol. 2001, 91, 21-40. https://doi.org/10.1016/S0377-8401(01)00228-0
https://doi.org/10.1016/S0377-8401(01)00228-0

[28] Sun, B.; Ricardo-da-Silva, J.M.; Spranger, I. Critical Factors of Vanillin Assay for Catechins and Proanthocyanidins. J. Agric. Food Chem. 1998, 46, 4267-4274. https://doi.org/10.1021/jf980366j
https://doi.org/10.1021/jf980366j

[29] Fettah, A.; Djouamaa, M.; Lamara, K. Courrier du Savoir 2018, 26, 357.

[30] Contreras-Calderón, J.; Calderón-Jaimes, L.; Guerra-Hernández, E.; García-Villanova, B. Antioxidant Capacity, Phenolic Content and Vitamin C in Pulp, Peel and Seed from 24 Exotic Fruits from Colombia. Food Res. Int. 2011, 44, 2047-2053. http://dx.doi.org/10.1016/j.foodres.2010.11.003
https://doi.org/10.1016/j.foodres.2010.11.003

[31] Molyneux, P. The Use of the Stable Free Radical Diphenylpi-crylhydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol. 2004, 26, 211-219.

[32] Pokorny, J.; Yanishlieva, N.; Gordon, M.H. Antioxidants in Food: Practical Applications; CRC press, 2001.
https://doi.org/10.1201/9781439823057

[33] Ngom, S.; Diop, M.; Mbengue, M.; Faye, F.; Kornprobst, J.; Samb, A. Composition chimique et propriétés antibactériennes des huiles essentielles d'Ocimum basilicum et d'Hyptis suaveolens (L.) Poit récoltés dans la région de Dakar au Sénégal. Afr. Sci. Rev. Int. Sci. Technol. 2014, 10, 109-117.

[34] http://dspace.univ-djelfa.dz:8080/xmlui/bitstream/handle/ 123456789/933/magister%20O%20basilicum.pdf?sequence=1&isAllowed=y (accessed Sep 4, 2019).

[35] Etude des activités antibactériennes et antioxydantes des extraits d'Ocimum basilicum (basilic) dans la région de Ain Defla - Sécheresse http://www.secheresse.info/spip.php?article79887 (accessed Sep 4, 2019).