1. JCCT
  2. Ch&ChT Vol. 19, No. 4, 2025
  3. Biologically Active Substances of Malva sylvestris L. Extracts as Sources of Safe Phytotherapeutic Agents

Biologically Active Substances of Malva sylvestris L. Extracts as Sources of Safe Phytotherapeutic Agents

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Olha Fedoryshyn1, Olena Yaremkevych1, Iryna Rusyn1, Ananiy Kohut1
Affiliation: 
1 Lviv Polytechnic National University, 12 S. Bandery St., Lviv 79013, Ukraine olha.m.fedoryshyn@lpnu.ua
DOI: 
https://doi.org/10.23939/chcht19.04.805
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Keywords: 
Malva sylvestris L. extracts
bioactive compounds
antioxidants
phenolic compounds
flavonoids
amino acids
Abstract: 
This study examines the content of major groups of biologically active substances (BAS) in extracts of Malva sylvestris L., a wild medicinal plant widely distributed across temperate ecosystems. Using established chemical analysis methods, reliable data were obtained for 40% and 70% aqueous-ethanol extracts regarding their BAS composition. The total phenolic content was determined spectrophotometrically using the Folin–Ciocalteu method, while the total flavonoid content was quantified by the aluminum chloride colorimetric assay. Comparative analysis revealed that the 40% extract contained higher levels of phenolic compounds (1.28 ± 0.02 mg/mL), flavonoids (0.77 ± 0.03 mg/mL), and amino acids (10.7 ± 0.01 mg/mL) than the 70% extract (0.88 ± 0.03 mg/mL, 0.35 ± 0.02 mg/mL, and 6.8 ± 0.01 mg/mL, respectively). Thin-layer chromatography additionally confirmed the presence of polysaccharides, quercetin, ascorbic acid, and anthocyanins in the analyzed samples. It was found that a 40% ethanol solution is a more effective extractant for polar compounds, whereas increasing the ethanol concentration to 70% reduces their solubility. The results confirm the potential of M. sylvestris L. extracts as a promising source of BAS for the development of safe phytotherapeutic agents.
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