Error message

  • Deprecated function: Unparenthesized `a ? b : c ? d : e` is deprecated. Use either `(a ? b : c) ? d : e` or `a ? b : (c ? d : e)` in include_once() (line 1439 of /home/science2016/public_html/includes/bootstrap.inc).
  • Deprecated function: Array and string offset access syntax with curly braces is deprecated in include_once() (line 3557 of /home/science2016/public_html/includes/bootstrap.inc).

Spectrophotometrical Study of Antioxidant Standards Interacting with 2,2-Diphenyl-1-picrylhydrazyl Radical

Touhami Lanez and Meriem Henni
Affiliation: 
University of El Oued, VTRS Laboratory, PO Box 789, 39000, El Oued, Algeria
DOI: 
https://doi.org/10.23939/chcht10.03.255
AttachmentSize
PDF icon full_text.pdf171.33 KB

Keywords:

Abstract: 
This work aims to study the interaction of four well known antioxidant standards with 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) using spectrophotometric assays. The binding parameters like binding constant and binding free energy of the free and DPPH• bound forms were determined. The determination is based upon the decrease in absorbance of the electronic absorption spectrum of an acetonitrile solution of DPPH• in the presence of gradually increasing amount of antioxidant standards.
References: 

[1] Gulcin I., Mshvildadze V., Gepdiremen A. et al.: Phytomedicine, 2006, 13, 343.
https://doi.org/10.1016/j.phymed.2005.03.009

[2] Villano D., Fernandez-Pachon M., Moya M. et al.: Talanta, 2007, 71, 230.
https://doi.org/10.1016/j.talanta.2006.03.050

[3] Boubekri C., Rebiai A. and Lanez T.: J. Fundament. Appl. Sci., 2012, 4, 164.
https://doi.org/10.4314/jfas.v4i2.6

[4] Blois M.: Nature, 1958, 26, 1199.
https://doi.org/10.1038/1811199a0

[5] Khelef A. and Lanez T.: Der Pharma Chemica, 2015, 7, 318.

[6] Rebiai A. and Lanez T.: J. Fundament. Appl. Sci., 2012, 4, 155.
https://doi.org/10.4314/jfas.v4i2.5

[7] Gulcin I.: Life Sci., 2006, 78, 803.
https://doi.org/10.1016/j.lfs.2005.05.103

[8] Magnani L., Gaydou E. and Hubaud J.: Anal. Chim. Acta, 2000, 411, 209.
https://doi.org/10.1016/S0003-2670(00)00717-0

[9] Sanchez J., Garcia R. and Cors M.: Pharmacol. Res., 2010, 2, 279.

[10] Shareef M., Reddy P., Gopinath S. et al.: Int. J. Innov. Res. Sci. Eng. Technol., 2014, 3, 10101.

[11] Le Bourvellec C., Hauchard D., Darchen A. et al.: Talanta, 2008, 75, 1098.
https://doi.org/10.1016/j.talanta.2008.01.007

[12] Pyrzynska K. and Pękal A.: Anal. Methods, 2013, 5, 4288.
https://doi.org/10.1039/c3ay40367j

[13] Xie J. and Schaich K.: J. Agric. Food Chem., 2014, 62, 4251.
https://doi.org/10.1021/jf500180u

[14] Awika J., Rooney L. Wu X. et al.: J. Agric. Food Chem., 2003, 51, 6657.
https://doi.org/10.1021/jf034790i

[15] Van den Berg R., Haenen G., Van den Berg H. et al.: Food Chem., 2000, 70, 391.
https://doi.org/10.1016/S0308-8146(00)00092-3

[16] Yu L., Halley S., Perret J. et al.: J. Agric. Food Chem., 2002, 50, 1619.
https://doi.org/10.1021/jf010964p

[17] Lanez T. and Hemmami H.: Sci. Stud. Res. Chem. Chemica. Eng. Biotech. Food Ind., in press.

[18] Shen Q., Zhang B., Xu R. et al.: Anaerobe, 2010, 16, 380.
https://doi.org/10.1016/j.anaerobe.2010.06.006

[19] Vijan L.: Analele Universita Nii din Bucuresti – Chimie, Anul 14 (serie noua), 1-2, 377.